Table 1.
Effect of StWIPK silencing on spreading of PVYN-GFP to upper non-inoculated leaves
| dpi | Systemically infected plants (%) | ||
|---|---|---|---|
| WIPK silenced | Empty vector | Positive control | |
| 14 | 0 | 14.3 | 33.3 |
| 18 | 0 | 14.3 | nd |
| 22 | 85.7 | 85.7 | 100 |
dpi, days post inoculation with PVYN-GFP; WIPK silenced, six plants were agroinfiltrated with TRV construct for silencing WIPK gene and infected with PVYN-GFP; Empty vector, six plants were agroinfiltrated with empty TRV construct and infected with PVYN-GFP; Positive control, three plants were infected with PVYN-GFP at the same time as WIPK silenced and empty vector plants; nd, not determined at this time point