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. Author manuscript; available in PMC: 2016 Oct 15.
Published in final edited form as: Cancer Res. 2016 Feb 26;76(8):2314–2326. doi: 10.1158/0008-5472.CAN-15-1364

Figure 2.

Figure 2

MCAM and its extracellular matrix binding partner LAMA4 are specific markers of endothelium in both renal and colorectal cancers. A, confirmation of cancer specific enrichment of MCAM and LAMA4 by RTqPCR on endothelial isolates from RCC, (n = 8), CRC, (n = 8), CRM, (n = 6) and associated healthy tissues (n = 8,8,6). Expression standardised to flotillin 2, confidence limits ± SEM, statistical analysis: Mann-Whitney U test (*** p < 0.001, ** p < 0.01, * p < 0.05). B, confirmation of cancer specific enrichment of MCAM and LAMA4 by immunohistochemistry (IHC). Representative images of kidney (arrows show glomeruli), RCC, colon and CRC stained for PECAM (endothelial marker), MCAM and LAMA4. Scale bar = 50 μm. C, analysis of marker expression in 18 common cancers and associated healthy tissues by IHC. The proportion of tissue of each type scored as exhibiting strong staining is shown. D, VEGF significantly induced MCAM expression in endothelial cells. HUVEC isolates (n=6) and HDMEC isolates (n=2 mixed isolates each in triplicate) were serum and growth factor starved for 12 hours, then cultured with serum depleted media ± 100 ng/ml recombinant human VEGF (hVEGF), MCAM expression was determined by western blot and RTqPCR. Confidence limits ± SEM, statistical analysis: Mann-Whitney U test, ** p < 0.01.