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. Author manuscript; available in PMC: 2016 Oct 15.
Published in final edited form as: Cancer Res. 2016 Feb 26;76(8):2314–2326. doi: 10.1158/0008-5472.CAN-15-1364

Figure 5.

Figure 5

A monoclonal anti-MCAM antibody specifically localises to murine RCC tumour vessels. A, triple immunofluorescent staining of a murine RCC (RENCA) tumour for; PECAM-1 (green), MCAM (red) and DAPI (blue). Scale bar = 25 μm. B & C, 20 μg of MCAM monoclonal antibody was intravenously injected into RENCA tumour bearing mice 1 hour prior to cull. The tumour and selected organs were collected. Frozen sections were stained with anti-rat IgGs (red), PECAM-1 (green) and DAPI (blue). B, the average optical density of fluorescence detected in the anti-rat IgG (red) channel, within regions of vascular (PECAM-1) staining was quantified. The tissue from two mice was assessed, with six regions of interest selected for each organ and mice (n=12), confidence limits ± SEM. C, representative images of MCAM monoclonal antibody localisation. Scale bar = 12.5 μm.