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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Plasmid. 2016 Feb 11;84-85:27–35. doi: 10.1016/j.plasmid.2016.02.003

Figure 2.

Figure 2

Deletion of MA4421 from the chromosome using pALD1. Schematics of the MA4421 genomic locus in the parental strain (A), and in the deletion mutant (B), are shown. (C) PCR results with oligos oNB274 and oNB319 showing deletion of MA4421 in two isogenic isolates. Gray box= MA4421 coding sequence. White boxes= DNA sequences upstream (“up”) and downstream (“down”) of the MA4421 gene that were used to create plasmid pALD1. Open arrowheads= annealing site of PCR oligos used to construct pALD1. Solid arrowheads= annealing site of PCR oligos used to screen for deletion of MA4421 on the chromosome. M= DNA size marker. Kb= kilobasepairs. The asterisks denote the expected amplicon sizes.