Table 1.
Strains used in this study
| NB# | Genotype | Purpose | Reference |
|---|---|---|---|
| E. coli strains | |||
| NB3 | 5α F' lacIq | parent | New England Biolabs |
| NB4 | 10β | parent | New England Biolabs |
| NB100 | 10β/pNB721 | Promotorless pac (opt) for conditional essentiality test of promoters in M. acetivorans | This study |
| NB101 | 10β/pNB722 | pac (opt) vector for homologous recombination repair of mutants, gene deletion by homologous recombination (marked) in M. acetivorans | This study |
| NB104 | 5α F' lacIq/pNB723 | Gene deletion by homologous recombination (markerless) in M. acetivorans | This study |
| NB128 | 5α F' lacIq/pNB724 | pac (opt) vector for homologous recombination repair of mutants, gene deletion by homologous recombination (marked) (unique SpeI restriction site) in M. acetivorans | This study |
| NB131 | 5α F' lacIq/pNB727 | operon insertion into ϕC31 attP site on the chromosome in M. acetivorans | This study |
| NB133 | 5α F' lacIq/pNB729 | Expression of native or tagged protein in M. acetivorans | This study |
| NB134 | 5α F' lacIq/pNB730 | Expression of native or tagged protein (unique BamHI restriction site) in M. acetivorans | This study |
| NB161 | 5α F' lacIq/pALD1 | MA4421 deletion by homologous recombination (markerless) in M. acetivorans | This study |
| NB224 | 5α F' lacIq/pSK1 | Expresses native UidA in M. acetivorans | This study |
| NB225 | 5α F' lacIq/pSK2 | Expresses strep-his-UidA-his-strep protein in M. acetivorans | This study |
| NB238 | 10β / pNB735 | Expresses native or tagged protein in M. acetivorans. Amino-terminal strep-his tag is cleavable with thrombin protease. | This study |
| NB239 | 10β/ pNB737 | Expresses native or tagged protein in M. acetivorans. Amino-terminal his tag is cleavable with thrombin protease. | This study |
| M. acetivorans strains | |||
| NB34 | Δhpt::ϕC31 int, attP | parent | [59] |
| NB218 | Δhpt::ϕC31 int, attP, ΔMA4421 | ΔMA4421 mutant | This study |
| NB231 | Δhpt::ϕC31 int, att:pSK1 | Expresses native UidA protein | This study |
| NB232 | Δhpt::ϕC31 int, att:pSK2 | Expresses strep-his-UidA-his-strep tagged protein | This study |