Fig. 2. Characterization of the AMPK activation status in CD11cAMPK mouse-derived pulmonary leukocytes during hookworm infection.

A-J) AMPKα–specific intracellular staining to compare CD11cAMPK (dotted line) and CD11c^Cre (gray histogram) for median fluorescence intensity expression levels in A, B) alveolar macrophages of naïve mice. C-E) MFI levels for AMPKα ICS at 9 dpi in autofluorescent alveolar macrophages and F) non-autofluorescent eosinophils (n=4/group). G-J) MFI expression levels in MHC-II^pos, H) CD103⁺ DC, I) interstitial macrophages (Mϕ), and J) CD11b DC (n=4). K) Data show pAMPKα levels in whole lung tissue lysates from WT C57BL/6 mice left un-treated (naïve) or at 3 dpi with 750 N.b. L₃ (n=3/group). L) Contour plot shows gating strategy for splenic CD4⁺ and CD8⁺ T cell gating. M-P) Representative MFI histograms from two independent experiments (n=4/group) show M, N) CD4⁺ T cells and O, P) CD8⁺ T cells from naive (dotted line) vs. 9 day N.b.-infected (red line) CD11c^Cre and CD11cAMPK mice. Mean ± SEM of MFI levels of AMPKα staining in gated Q) CD4⁺ T cells and R) CD8⁺ T cells from 4 mice/group. All data represents 2-3 independent experiments. *p<0.05, **p<0.01, ***p<0.001