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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Dev Biol. 2016 Mar 16;414(1):100–107. doi: 10.1016/j.ydbio.2016.03.009

Figure 1. Generation and verification of the Gia mutant.

Figure 1

(A) A Gia null mutant was generated by FRT-mediated recombination between transposable element-based insertions flanking the Gia/Mthl5 locus. (B) Genomic PCR performed on DNA extracted from wild type (WT) and GiaAD5 homozygous mutant (Gia−/−) embryos produced the anticipated amplification product to confirm the chromosomal deletion. (C) RT-PCR analysis confirmed the absence of Gia mRNA in GiaAD5 homozygous mutant embryos, compared to wild type and GiaAD5 heterozygous mutant (Gia−/+) embryos. (D, E) In situ hybridization detected Gia mRNA in wild type but not in GiaAD5 homozygous mutant embryos.