Figure 2.
24-hour IL-10 treatment enhances FcεRI-mediated cytokine production in peritoneal derived mouse or human skin mast cells. (A) Mouse peritoneal derived mast cells and (B) human skin mast cells were incubated with IgE and 50ng/ml of IL-10 for 24 hours prior to antigen-induced IgE crosslinking (XL) for 16 hours. Data are means ± SEM of (A) three independent experiments done in triplicates or (B) cells from 5 donors tested 6 times. Cytokines were measured using ELISA.