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Strains selected for genome shuffling. In the phase 1, strains in bold correspond to the selected strains to carry out genome shuffling steps on. First- and second-generation shuffled strains are indicated in bold next to their parents. Self-shuffles mean that the shuffling protocol was carried out on cells belonging to a unique NTG mutant corresponding to a unique population of cells. Concentrations of isopropanol used for selection of mutants on plates at each generation is indicated
