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Isopropanol toxicity test plate assay of Clostridium beijerinckii NRRL B593 wild type and mutants. F0 strains are strains resulting from chemical mutagenesis. F1 strains correspond to strains obtained after the first round of genome shuffling. F2 strains correspond to the strains obtained after the second round of genome shuffling. mCGM plates containing 0, 35, 40, 45, and 50 g/L isopropanol were used for growth of the colonies. Plates were incubated at 37 °C for 48 h
