Table 1.
Short characteristic of the proteins showing different abundance between primary, recurrent tumors and metastases (2D comparative proteome profiling)
| Spot no. | Protein identification | Accession (SwissProt) | Sequence coverage (%) | Score | Peptides matched | Theoretical MW (kDa) | Theoretical pI | Fold change (compared to primary tumors) | |
|---|---|---|---|---|---|---|---|---|---|
| Recurrent | Metastases | ||||||||
| 1 | Annexin V | ANXA5_HUMAN | 51 | 163 | 16 | 35.9 | 4.94 | 0.83 | 1.63 |
| 2 | Annexin III | ANXA3_HUMAN | 30 | 108 | 10 | 36.5 | 5.63 | 0.42 | 0.50 |
| 3 | Calreticulin | CALR_HUMAN | 51 | 172 | 19 | 48.2 | 4.29 | 0.95 | 1.74 |
| 4 | 60 kDa heat shock protein | CH60_HUMAN | 49 | 177 | 28 | 61.1 | 5.7 | 0.72 | 0.54 |
| 5 | Heat shock 70 kDa protein 9 | GRP75_HUMAN | 41 | 170 | 23 | 73.9 | 5.87 | 0.71 | 0.64 |
| 6 | Protein disulfide-isomerase A3 | PDIA3_HUMAN | 32 | 85 | 12 | 57.1 | 5.98 | 0.84 | 0.52 |
| 7 | Inorganic pyrophosphatase PPA1 | IPYR_HUMAN | 52 | 161 | 12 | 33 | 5.54 | 1.02 | 2.10 |
Fold change <1 means downregulation; significant differences are bolded