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The impact of changes to the invasion oligonucleotide (IO) seeding region nucleotide composition on the average detection time for the SIBA influenza A assay. The impact of the seeding region a length and b composition (for a ten nucleotide seeding region). The results are presented as the average detection time from eight replicate assays (standard deviation included). The detection time reported was the time at which the probe fluorescent signal exceeded the background signal
