Table 3.
Binding kinetics and affinities for the Tg antibodies E40, E44, E45, E47, I15, and I24
| MAb | SPR analysis | Estimates from equilibrium analysis | ||
|---|---|---|---|---|
|
K
a
[M−1 s−1] |
K
d
[M−1 s−1] |
K
D
[M] |
K
D
[M] |
|
| E40 | 3.32 × 105 | 3.69 × 10−4 | 1.1 × 10−9 | 1.2 × 10−9 |
| E44 | 3.14 × 105 | 1.29 × 10−4 | 4.1 × 10−10 | 3 × 10−10 |
| E45 | 4.99 × 105 | 1.98 × 10−4 | 4.0 × 10−10 | 2 × 10−10 |
| E47 | 2.95 × 105 | 3.23 × 10−5 | 1.1 × 10−10 | 6 × 10−10 |
| I15 | 2.46 × 105 | 6.29 × 10−5 | 2.6 × 10−10 | 3 × 10−10 |
| I24 | 4.24 × 105 | 2.15 × 10−4 | 5.1 × 10−10 | 2 × 10−10 |