Table 1.
Effect of different compounds on endoglucanase and beta-glucosidase relative activity of the dialyzed crude extract expressed as mean values ± SD. NT = not tested; ND = not detected. The asterisks represent significant differences against the control with p < 0.05 according to Student's t-test for means.
| Compound | Conc. (mM) | Endoglucanases | β-Glucosidases | ||
|---|---|---|---|---|---|
| Rel. act. SSC (%) | Rel. act. SmC (%) | Rel. act. SSC (%) | Rel. act. SmC (%) | ||
| PVA | 10 | 120.9 ± 2.8∗ | 110.2 ± 10.4 | NT | NT |
| Isopropanol | 10 | 116.7 ± 0.7∗ | 74.1 ± 1.6∗ | 106.4 ± 4.1 | 92.9 ± 5.0 |
| NaCl | 10 | 113.3 ± 0.7∗ | 90.3 ± 3.9∗ | 95.9 ± 3.9 | 71.5 ± 2.5∗ |
| DTT | 10 | 112.5 ± 0.9∗ | 121.4 ± 4.9∗ | 86.4 ± 2.6∗ | 92.9 ± 3.6 |
| PMSF | 1 | 110.2 ± 0.3∗ | 110.2 ± 11.2 | 116.4 ± 4.8∗ | 101.2 ± 6.4 |
| Glucose | 10 | 109.5 ± 0.9∗ | 89.2 ± 12.5 | 59.4 ± 6.1∗ | 69.0 ± 1.1∗ |
| DMSO | 10 | 109.3 ± 2.3∗ | 108.9 ± 18.8 | NT | NT |
| Acetone | 10 | 101.0 ± 1.2 | 95.9 ± 8.4 | 92.7 ± 3.4 | 106.1 ± 5.4 |
| Triton X-100 | 10 | 101.2 ± 1.3 | 114.3 ± 16.7 | NT | NT |
| Ethanol | 10 | 101.0 ± 2.9 | 71.4 ± 3.7∗ | 94.7 ± 11.2 | 85.0 ± 5.3 |
| MgCl2 | 10 | 89.7 ± 2.1∗ | 35.1 ± 3.4∗ | 99.3 ± 3.6 | 76.7 ± 1.0∗ |
| PEG 8000 | 3 | 85.5 ± 0.4∗ | 93.7 ± 5.3 | NT | NT |
| PEG 3350 | 10 | 112.8 ± 1.7∗ | 97.1 ± 7.6 | NT | NT |
| CaCl2 | 10 | 68.3 ± 1.0∗ | 31.4 ± 2.5∗ | 79.5 ± 7.6∗ | 100.7 ± 2.0 |
| SDS | 10 | 57.0 ± 0.8∗ | 82.1 ± 4.5∗ | 79.9 ± 1.6∗ | 80.8 ± 2.9∗ |
| EDTA | 10 | 84.5 ± 2.7∗ | 74.8 ± 13.1∗ | 89.2 ± 8.9 | 94.0 ± 2.5 |
| AlCl3 | 10 | 30.5 ± 0.5∗ | 1.7 ± 0.7∗ | 26.9 ± 10.7∗ | 21.2 ± 0.7∗ |
| MnCl2 | 10 | 14.2 ± 1.4∗ | 113.0 ± 3.1∗ | 177.0 ± 11.7∗ | 105.0 ± 1.7 |
| FeCl3 | 10 | 11.0 ± 0.4∗ | ND∗ | 21.3 ± 14.1∗ | ND∗ |