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. 2016 Apr 5;24(5):978–990. doi: 10.1038/mt.2016.41

Figure 4.

Figure 4

SENCR overexpression does not impact on human embryonic stem cells (hESC) pluripotency. (a) Schematic representation of constructed recombinant lentivirus (LV) vectors harbouring SENCR sequence under SFFV promoter. LTR, long-terminal-repeat; Ψ = Psi, packaging signal; RRE, Rev response element; cPPT, central polypurine tract; SFFV, promoter of spleen focus-forming virus; wPRE, Woodchuck hepatitis virus posttranscriptional regulatory element. (b) Experimental design used to transduce pluripotent H9 hESC. (c–f) All of measures were performed 3 days postinfection and LV-SENCR-infected group is compared to LV-control infected group. (c,d) Gene analysis for SENCR expression (c) and pluripotent/germ-layer genes (d) by quantitative reverse transcription-PCR (all error bars = RQmax). (e) Representative fluorescence-activated cells sorting and quantification of immunophenotype profile analysis for TRA-1-60 and SSEA-3 (all error bars = SEM). (f) Image representing morphology of H9 hESC colony (scale bar 125 μm). (a–f) n = 3, Student's t-test. ***P < 0.005.