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. Author manuscript; available in PMC: 2017 Apr 15.
Published in final edited form as: Cancer Res. 2016 Feb 15;76(8):2419–2431. doi: 10.1158/0008-5472.CAN-15-1691

Figure 2. Genetic ablation uncovers the stemness-suppressive function of Klf4 in mouse model.

Figure 2

(A–B) WB analysis of KLF4 expression in parental 723G/924G (Klf4loxp/loxp) and derived 723K/924K (Klf4−/−) cells (Ai & Bi), and their spheroid-forming abilities were determined in tumorsphere culture (Aii–iii & Bii–iii).

(C–D) Paired 723G/723K and 924G/924K cells carrying the luciferase reporter gene were intravenously injected into mice through the tail vein (n=8/group), and lung tumor metastasis was monitored by in vivo bioluminescence imaging (representative photos, Ci & Di), and the metastatic nodules visible on the surface of the lungs were accounted at the end of experiment (representative images, Cii & Dii; quantitative data, Ciii & Diii).

(E–F) 723G and 723K cells were subcutaneously injected into the abdominal flanks of mice (n=5/group), respectively, and representative images of tumor growth (Ei) and the average tumor weight measured (Eii) at the end of experiment are showed. Similar results were obtained using the paired 924G/924K cells (Fi & Fii). (*P<0.05; **P<0.01).