Table 2.

Hemadsorption inhibitory activity of patient and non-patient sera.

Serum No.a	Antibody titerb	p1 gene types detected from patient sputac	Hemadsorption (HA) inhibitory activityd
			M129 (Type 1)		FH (Type 2)
			1/5	1/10	1/5	1/10
1	1280	1	+	±	–	–
2	2560	1	+	±	±	–
3	1280	1	+	+	+	±
4	>2560	1	+	+	–	–
5	>2560	2	±	–	+	±
6	>2560	2	±	–	+	±
7	1280	2	±	–	±	–
8	>2560	1	+	+	–	–
9	1280	2	±	–	+	±
10	>2560	1	+	–	–	–
11	ND	NT	–	–	–	–
12	ND	NT	–	–	–	–

^aSerum number corresponds to the lane number of Figure 5B. ^bAntibody titer was measured with Serodia Myco II kit (Fujirebio, Tokyo, Japan); ND, not determined. ^cDetection and typing of the p1 gene was performed by the nested PCR method as a part of previous study (Kenri et al., 2008); NT, not tested. ^dFive to ten colonies of M. pneumoniae M129 (type 1) or FH (type 2) strains were formed on PPLO agar cast in a 24-well microplate (1.5 ml PPLO agar per well). Colony-forming wells were soaked with 1 ml of phosphate-buffered saline (PBS) for 5 min at room temperature. After removal of PBS, 0.1 ml of diluted patient serum (fivefold or 10-fold dilution by PBS) was added to the well and incubated for 20 min at 37°C. After removal of the patient serum, 0.5 ml of a diluted sheep red blood cell (RBC) suspension (100-fold dilution by PBS) was added to the well and incubated for 20 min at 37°C. After incubation, excess RBCs were removed by washing with 1 ml of PBS three times. The state of hemadsorption of M. pneumoniae colonies was evaluated by microscopic observation. +: complete hemadsorption inhibition (no adsorption of RBCs was observed); ±: partial inhibition (partial adsorption of RBCs was observed); –: no inhibition (colonies were fully covered by RBCs; Kenri et al., 2006b).