Figure 5.

Inhibition of LRRK2 prevents effect on Ca_V2.1 current density. (A) Mean I-V relationships of peak Ca²⁺ current density from HEK293 cells co-transfected with Ca_V2.1 and LRRK2 pre-treated with vehicle (white triangles) or the LRRK2 inhibitor LRRK2-IN-1 (gray circles; n = 10 cells from four independent experiments). Also shown are mean I-V relationships of cells co-transfected with Ca_V2.1 channels and G2019S pre-treated with vehicle (crosses) or LRRK2-IN-1 (black circles; n = 10 cells from four independent experiments). (B) LRRK2-IN-1 treated cells had a significantly reduced peak Ca²⁺ current density compared to vehicle (where ** indicates p < 0.01) while peak Ca²⁺ current density in G2019S cells was still significantly elevated over LRRK2 cells pre-treated with vehicle (where * indicates p < 0.05) and significantly reduced by LRRK2-IN-1 pre-treatment (where ** indicates p < 0.01; one-way ANOVA). (C) LRRK2-IN-1 pre-treatment (gray circles) had no effect on Ca_V2.1 current density in the absence of exogenous LRRK2 expression compared to vehicle treated controls (black circles; n = 10 cells from four independent experiments). (D) Peak Ca²⁺ current density was comparable between LRRK2-IN-1 pre-treated cells compared to vehicle controls.