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. 2016 May 23;7:748. doi: 10.3389/fmicb.2016.00748

FIGURE 1.

FIGURE 1

Effect of cobalamin on ROS generation. Cytoplasmic ROS content was estimated by measuring activation of the fluorescent-probe H2DCFDA in Leptospirillum CF-1 cells treated with oxidative agents as indicated under Section “Materials and Methods.” Fluorescence values were expressed as relative fluorescence units (RFU). Bars represent the average of three independent experiments ± standard deviations. P < 0.05, ∗∗P < 0.01.