FIGURE 1.

Effect of cobalamin on ROS generation. Cytoplasmic ROS content was estimated by measuring activation of the fluorescent-probe H₂DCFDA in Leptospirillum CF-1 cells treated with oxidative agents as indicated under Section “Materials and Methods.” Fluorescence values were expressed as relative fluorescence units (RFU). Bars represent the average of three independent experiments ± standard deviations. ^∗P < 0.05, ^∗∗P < 0.01.