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Effect of cobalamin on superoxide dismutase activity. The activity was measured by following the inhibition of NBT-reduction at 560 nm as described under Section “Materials and Methods.” Data represent the average of two independent experiments (lines on top of bars indicate value ranges). One unit (U) is defined as the amount of enzyme necessary to decrease the reference rate to 50% of maximum inhibition. ∗∗P < 0.01.
