Figure 2.

The effect of Dic on PregS‐induced Ca²⁺ responses of HEK‐TRPM3s cells. (A–C) PregS at 10 μmol/L (for HEK‐TRPM3₁₃₂₅ [A] and HEK‐TRPM3‐3 [B]) or 30 μmol/L (for HEK‐TRPM3‐9 [C]) was applied twice and 30 μmol/L Dic was added before the second application of PregS. (D) Change in PregS‐induced Ca²⁺ response by Dic at a concentration range between 1 and 100 μmol/L is summarized as concentration–response relationships. The amplitude of change in the second PregS‐induced Ca²⁺ response with Dic was normalized to that in the first without Dic (square symbols, six independent experiments for TRPM3₁₃₂₅; circle symbols, six independent experiments for TRPM3‐3; triangle symbols, six independent experiments for TRPM3‐9). Data were fitted to a sigmoid curve to determine the apparent IC ₅₀ of Dic against PregS‐induced Ca²⁺ response of each TRPM3 isoform. Dic, diclofenac; PregS, pregnenolone sulfate; HEK, human embryonic kidney; TRPM, transient receptor potential melastatin.