Figure 3.

(A–C) Change in current amplitude of TRPM3₁₃₂₅ at −80 mV and +80 mV was plotted against time (A). I‐Vs evoked by ramp voltage command pulses were also shown in the control and with or without 10 and 30 μmol/L Dic in the presence of 30 μmol/L PregS (B). Ramp voltage command pulses for 400 msec were applied every 10 sec at a holding potential of −10 mV. Comparison of relative change in PregS‐evoked current amplitude by 10 and 30 μmol/L Dic in HEK‐TRPM3₁₃₂₅ cells (C). Peak current amplitude at −80 mV and +80 mV was pooled and summarized in a bar graph. Bars represent the mean ± SEM from eight cells. (D–F) As for (A–C) except TRPM3₁₃₂₅ to TRPM3‐3. Bars represent the mean ± SEM from four to six cells. (G–I) As for (A–C) except TRPM3₁₃₂₅ to TRPM3‐9. Bars represent the mean ± SEM from five cells. ** versus without Dic. ## versus with 10 μmol/L Dic. TRPM, transient receptor potential melastatin; Dic, diclofenac; PregS, pregnenolone sulfate; HEK, human embryonic kidney.