Figure 5.

No effects of a glycolic acid ester of Dic (Afc) on TRPM3 and Dic on TRPM2, TRPM8, and TRPV4. (A) Chemical structures of Dic and Afc. (B and C) Effects of 100 μmol/L Afc on PregS‐induced Ca²⁺ response in HEK‐TRPM3₁₃₂₅ cells: a representative trace (B) and summary of change in Ca²⁺ response in the absence and presence of Afc (C, three independent experiments). (D) Summary of effects of 100 μmol/L Dic on the Ca²⁺ response to TRPM2 (four independent experiments), TRPM8 (five independent experiments), and TRPV4 (four independent experiments). Hydrogen peroxide (H₂O₂; 1 mmol/L), menthol (Men; 100 μmol/L), and GSK1016790 (GSK; 3 nmol/L) were applied to evoke TRPM2‐, TRPM8‐, and TRPV4‐mediated response, respectively. Dic, diclofenac; Afc, aceclofenac; TRPM, transient receptor potential melastatin; TRPV, transient receptor potential vanilloid; PregS, pregnenolone sulfate; HEK, human embryonic kidney.