Figure 7.

Channel conductance and open probability of TRPM3₁₃₂₅ with or without Dic. (A) A representative single‐channel current trace in an outside‐out patch excised from a HEK‐TRPM3₁₃₂₅ cell. The channel activity was increased by 30 μmol/L PregS and thereafter 10 and 30 μmol/L Dic were applied. During application of PregS and PregS plus Dic, the holding potential was changed from +70 to +80, +90, and +100 mV to determine the channel conductance. In lower panels, traces at +70 and +90 mV were shown in a higher time resolution (B). The “c” indicates the channel close level. I‐Vs of PregS‐induced single‐channel currents with or without Dic (C, PregS; five cells, +10 μmol/L Dic; four cells +30 μmol/L Dic; four cells). The channel conductance was calculated to fit a set of data in each cell to a linear regression line. (D) Amplitude histograms at +80 mV for 5 sec in the presence of 30 μmol/L PregS, 30 μmol/L PregS plus 10 μmol/L Dic, and 30 μmol/L PregS plus 30 μmol/L Dic from the same cell as shown in (A). The bin width is 0.163 pA. (E) The open probability of PregS‐evoked single‐channel currents was summarized before and during application of 10 and 30 μmol/L Dic. Bars represent the mean ± SEM from three to four cells. * versus without Dic. TRPM, transient receptor potential melastatin; Dic, diclofenac; HEK, human embryonic kidney; PregS, pregnenolone sulfate.