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TGF-β1 downregulates H3K9me2/3 levels at p21 gene promoter in RMC. (a) Map showing locations of p21 promoter primer used for ChIP-QPCRs. TSS: transcription start site; SBE: Smad binding elements. (b) ChIP assays performed with H3K9me2 and H3K9me3 antibodies normalized to input DNA. Results are expressed as fold over control (mean ± SEM; # P < 0.05 versus ctrl, n = 3).
