Table 2.

On‐rate, off‐rate and dissociation constants (K _d) of uPA‐SPD variants from PAI‐1 measured by SPR

uPA‐SPD variants	k on (105/Ms)	k off (10−4/s)	K d (nM)
S195A‐C122A‐N145Q	7.76 ± 0.60	12.1 ± 0.5	1.57 ± 0.20
S195A‐C122A‐N145Q‐G37bR	23.0 ± 5.4	6.98 ± 0.32	0.32 ± 0.06
S195A‐C122A‐N145Q‐Y60bK	17.0 ± 2.4	6.20 ± 0.25	0.37 ± 0.06
S195A‐C122A‐N145Q‐R217L	13.3 ± 0.4	8.40 ± 0.56	0.63 ± 0.03
S195A‐C122A‐N145Q‐G37bR‐Y60bK	23.5 ± 16.1	11.4 ± 4.2	0.83 ± 0.53
S195A‐C122A‐N145Q‐G37bR‐R217L	41.1 ± 2.0	6.27 ± 1.02	0.15 ± 0.02