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. 2016 May 23;6:26521. doi: 10.1038/srep26521

Figure 6. MTH1 inhibition does not affect cancer cell growth.

Figure 6

(a,b) Overexpression of MTH1 did not rescue cells from MTH1 inhibitors-induced cell death. HeLa cells transiently overexpressing human MTH1 and the control counterparts were treated with MTH1 inhibitors for 84 h. The expression levels of MTH1 proteins in both cells were analyzed by western blot (a). The cell viability was examined by WST-8 assay. Data are shown as mean ± s.d. There was no statistically significant difference between the viability of MTH1-overexpressing HeLa cells and that of the control counterparts following treatment with MTH1 inhibitors at all concentrations tested (ANOVA followed by Games-Howell test) (b). (c–f) Knockdown of MTH1 did not affect cell survival. HeLa cells were transfected with 20 nM siRNA for 72 h, and the knockdown of MTH1 was analyzed by western blot (c). Cell growth of HeLa cells transfected with 20 nM siRNA for the indicated times (d,e). Data are shown as mean ± s.d. Statistical analysis was performed using Student’s t-test. Cell cycle analysis of HeLa cells transfected with 20 nM siRNA for 96 h (f).