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. 2016 May 23;6:26547. doi: 10.1038/srep26547

Figure 1. Chemical genetic screen of the proliferation of fetal NP cells.

Figure 1

(A) Scatter plot of all the compounds screened on the two types of fetal NP cells and the control astrocyte cells. Five pharmacological classes were identified to increase cell proliferation above the 3σ threshold (dashed lines) from the control mean (solid lines). Blue, GSK3i; red, ERKi; green, β adrenoceptor agonists; pink, A1/A3 adenosine receptor agonists; orange, RHOi. (B) Proliferation assay results of a comprehensive collection of the five pharmacological classes. Results were normalized and presented in heat maps. Consistent promoting effects were observed for the structurally diverse compounds. The effects of ERKi and GSK3i were the strongest. (C) Dose-response curves (mean ± s.d., n = 3) of GSK3i BIO, ERKi U0126, RHOi Y27632, β adrenoceptor agonist norepinephrine (NE), and A1/A3 adenosine receptor agonist cyclopentyladenosine (CPA). The results on Rat CX cells were shown in red and control astrocytes shown in blue. ERKi and GSK3i were confirmed to be the two most potent classes of chemicals. Except for RHOi, the promoting effects of the other four chemicals were not detected on the control astrocytes.