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. 2016 May 23;6:26547. doi: 10.1038/srep26547

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) ERKi U0126 failed to trigger significant Erk-Akt crosstalk in adult NP cells, hES-derived NP cells, brain tumor U87 cells, and breast cancer MCF-7 cells in which Erk-Akt crosstalk has been extensively studied. The opposite crosstalk can be triggered by PMA in adult NP, hES-derived, and MCF-7 cells. (B) U0126 treatment failed to promote the proliferation of the four cell types. An inhibitory effect was observed in adult NP and U87 cells. Mean ± s.d., n = 4. *P < 0.05. ns, not significant. All gels were run under the same experimental condition. Cropped images were shown in the figure. Full length blots are included in the Supplementary Information.