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. 2016 May 23;6:26436. doi: 10.1038/srep26436

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Assembling of adipose-derived stem cells (ASC) with DP cells was developed by; mixing ASC with DP cells (MA-DPS) for co-culture or sequential seeding of DP cells and then ASC to form a core-shell sphere (CSA-DPS) in 96-well CCM (A). In the phase images of CSA-DPS, the ASCs were added after the DP sphere formed at Day 2 and can assembled outside the DP sphere at Day 3. The ASCs was labeled with DiI (red color) to visualize the cell arrangement with immunofluorescent staining of Hey 1 (green color) after assembling for 3 days. The confocal images from different Z sections revealed the cellular arrangements in MA-DPS and CSA-DPS (B). The core-shell culture of ASCs with DP cells (CSA-DPS) enhanced the expressions of VCAN and Hey1, but the mixed culture decreased the expression of DP markers in MA-DPS (C). Scale bar = 100 μm