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. 2016 May 23;6:26669. doi: 10.1038/srep26669

Figure 2. Corner-swimming preference of sperm in square microchannels.

Figure 2

Cross-sectional distribution of 1176, 1457, and 1188 bull sperm in square microchannels with side-lengths of (a) 50 μm, (b) 100 μm, and (c) 400 μm, respectively. For comparison, the 50 μm and 100 μm channels are shown to scale inset in the bottom left corner of the 400 μm channel plot. The color bar represents the relative density of sperm in each graph. (d) Percentage of bulk swimmer (PBS), percentage of wall swimmer (PWS), and percentage of corner swimmer (PCS) sperm as a function of microchannel size. Wall-swimming sperm values do not include corner swimmers. Each point represents experiments with at least three samples with a minimum of 874 sperm imaged in each experiment. Values are reported as mean ± s.d. (e) Histograms of sperm distance from the closest corner for each case. A total number of 800 sperm is included in each case.