Table 1. Width measurements of microbes.
Specimen | Sample preparation | Electron Imaging | Width (nm) | N | Relative Size |
---|---|---|---|---|---|
Salmonella Senftenberg | Ionic liquid | SEM | 627.1 ± 50.9 | 193 | – |
Salmonella Senftenberg | Sputter coating | SEM | 564.9 ± 44.4 | 185 | 90.1% |
Salmonella Senftenberg | Negative Stain | TEM | 563.6 ± 43.5 | 174 | 89.9% |
Leptospira biflexa | Ionic liquid | SEM | 156.2 ± 15.8 | 153 | – |
Leptospira biflexa | Sputter coating | SEM | 129.7 ± 14.1 | 158 | 83.0% |
Leptospira biflexa | Negative Stain | TEM | 126.8 ± 17.0 | 164 | 81.8% |
Ebola virus | Ionic liquid | SEM | 98.5 ± 10.2 | 123 | – |
Ebola virus | Sputter coating | SEM | 85.6 ± 8.4 | 141 | 86.9% |
Ebola virus | Negative Stain | TEM | 82.8 ± 5.0 | 146 | 84.1% |
The effect of sample preparation methods on microorganism size. N = number of measurements.