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. 2016 May 23;6:26506. doi: 10.1038/srep26506

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(a) Scheme of the LbL film-deposition. Positively charged PEI deposited on the glass slide treated with Piranha solution ahead of schedule. Steps 1 and 3 represent the adsorption of negatively charged colloidal gold-collagen nanoconjugates and positively charged TPPAc-PLL, steps 2 and 4 are washing steps. (b) UV-vis absorption spectra of the gold-collagen nanoconjugate/TPPAc-PLL films assembled on a glass slide with increasing number of deposition cycles. SEM (c) and AFM (d) images showing the surface morphology of a (gold-collagen nanoconjugates/TPPAc-PLL)₅ film. (e) AFM images with section analysis of the (gold-collagen nanoconjugates/PLL-TPPAc)₅ film, indicating a film thickness of 9.8 nm.