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. 2016 May 23;6:26463. doi: 10.1038/srep26463

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Fresh and cryo-MSC, both passage 4 MSC from donor 7083, were cultured for 48 hours in reduced serum media with or without stimulation by TNF-α/IFN-γ, the media was collected, and screened for 40 growth factors. Of the 40 proteins, 14 were detectable in at least 2 of the tested conditions and are reported here. Values represent measured concentration after background subtraction (unconditioned control media). A full table of values is available in Supplemental Figure 1. (A) The concentration of each growth factor produced by fresh and cryo-MSC over 48 hours in unstimulated conditions. (B) Fold change in concentration in unstimulated MSC, [Cryo]/[Fresh] (C): The concentration of each growth factor produced by fresh and cryo-MSC over 48 hours in stimulated conditions. (D) Fold change in concentration in stimulated MSC, [Cryo]/[Fresh]. Dashed line at 1 corresponds to no-change between groups.

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