Figure 5. Cryopreserved MSC do not persist in the eye following ischemia/reperfusion injury.

Human cells, identified by human specific Tra 1–85 immunostaining and counterstained with DAPI were undetectable in (A) untreated eyes and rare in (B) treated eyes at 3 days, white arrows indicate positive cells atop the ganglion cell layer. (C) Quantitative PCR for human genomic DNA remaining in mouse retina seven days after transplantation. Human DNA was not detected in any of the tested eyes (n = 5, mean ± SD). Data normalized as % of injected as 30,000 MSC were delivered to each eye. Donor 7083 used for all mice. Positive control is DNA extracted from 1,670 MSC, corresponding to 5.6% of the total injected MSC). GCL: Ganglion cell layer, INL: Inner Nuclear Layer, ONL: Outer Nuclear Layer.