Figure 4. Characterization of the lipid-rich strain B₁ZFeL.

(a) Fluorescence images of BODIPY^505/515-stained WT (Z strain) and B₁ZFeL cells in the aerobically heterotrophic culture. (b,c) Histogram of the BODIPY^505/515-stained WT and B₁ZFeL cells. The cells without (b) and with (c) hypoxic incubation were stained with BODIPY^505/515 and analyzed by FACS analysis. The fluorescence intensity is the strength of the light that passed through the 529/28 bandpass filter. The three histograms represent the results of independent cultures for each strain. The values in the plots are the average fluorescence value of each strain with the standard error of the mean N = 3. (d,e) Lipid (d) and paramylon (e) contents of the WT and B₁ZFeL in the heterotrophic culture with and without hypoxic incubation. N = 3. *p < 0.05, **p < 0.005, t-test with Bonferroni’s correction.