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. 2016 May 23;6:26480. doi: 10.1038/srep26480

Figure 1. Pyk2 regulates VEGF-induced angiogenic events in HRMVECs.

Figure 1

(a) Quiescent HRMVECs were treated with and without VEGF (40 ng/ml) for the indicated periods and cell extracts were prepared. Equal amounts of protein from control and each treatment were analyzed by Western blotting for pPyk2 levels using its specific antibodies. The blot was reprobed for total Pyk2 or β-tubulin for normalization. (b) HRMVECs were transduced with Ad-GFP or Ad-dnPyk2 (40 moi), growth-arrested, treated with and without VEGF (40 ng/ml) for 10 min, cell extracts were prepared and equal amounts of protein from control and each treatment were analyzed for pPyk2 levels and the blot was reprobed for total Pyk2 or β-tubulin to show the over expression of dnPyk2 or normalization. (c–f) All the conditions were the same as in panel b except that after growth-arrest, cells were subjected to VEGFA-induced migration (c), DNA synthesis (d), sprouting (e), or tube formation (f) assays. The bar graphs represent quantitative analysis of three independent experiments. The values are presented as Mean ± SD. *p < 0.01 vs control or Ad-GFP; **p < 0.01 vs Ad-GFP ± VEGFA.