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. 2016 May 23;6:26150. doi: 10.1038/srep26150

Figure 1. HBV infection inhibited expression of RIG-I like receptors.

Figure 1

(a) Western blot analysis of RIG-I, MDA-5, IFIT1 and RIG-G expression levels in HepG2 and HepG2.2.15 cells. (b) Western blot assay of RIG-I, MDA-5 and RIG-G protein levels in liver paracancerous tissues from 4 HBV+, 3 HBV− HCC and 1 intrahepatic cholangiocarcinoma (ICC) patients. (c) HepG2 cells were co-transfected with 0.05 μg RIG-I CARD, IFN-β–luc and pRL-TK reporter vectors, as well as RIG-G constructs or 100 nM RIG-I/RIG-G siRNA. After 36 hours, luciferase activity was measured. (d) RIG-I CARD/RIG-G constructs (0.5 μg/ml) and pAAV/HBV1.2 plasmid were co-transfected into HepG2 cells, together with the IFN-β–luc and pRL-TK reporter vectors, and luciferase activity was measured 36 hours later. (e) RIG-I CARD/RIG-G constructs (0.5 μg/ml) and pAAV/HBV1.2 plasmid (0.5 μg/ml), as well as 100 nM RIG-I siRNA were co-transfected into HepG2 cells, together with the IFN-β–luc and pRL-TK reporter vectors, and ELISA method was used to measure IFN-β production 36 hours later. Data are expressed as the mean ± SD from at least 3 independent experiments. *p < 0.05: versus the control vector–transfected group.