FIG. 2.

The effect of DATS on cycle arrest and apoptosis of BGC823 cells. (A) FACS analysis of the cycle of BGC823 cells after 12 h of treatment with DATS at the indicated concentrations. Left panel: representative histograms with the percentage of cells in each cell cycle phase (Left red peak: G0/G1; right red peak: G2/M; hatched peak: S; Coefficient of variation of G1 peak: % CV). Right panel: quantitative analysis of G2/M phase rate from three experiments shown as the mean ± standard deviation, **p < 0.005, DATS at indicated concentrations versus control. (B) FACS analysis of apoptosis in BGC823 cells after 12 h of treatment with DATS at 40 μM. Left panel: representative FACS plots. Right panel: quantitative data of apoptotic rate presented as the mean ± standard deviation (n = 3), *p < 0.05. (C) Western blotting of CCNB1 and a-CASP3 protein in BGC823 cells treated with DATS (40 μM, 12 h) or saline. Results are representative of four independent experiments. (D) Immunofluorescence staining of CCNB1 and a-CASP3 in BGC823 cells treated with DATS (40 μM, 12 h) or saline. Nuclei shown by DAPI staining (blue). Shown are results from one of two comparable experiments. Scale bars, 10 μM. a-CASP3, activated caspase-3.To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/ars