FIG. 3.

The effect of DATS on NF-κB activity in GC cells. (A) RT-PCR. (B) Western blotting of mRNA and protein of NF-κB pathway genes in BGC823 cells after 12 h of treatment with DATS at the indicated concentrations. (C) Western blotting of NF-κB p65 subunit from nuclear and cytoplasmic extracts of BGC823 cells treated with 40 μM DATS at the indicated time points. β-Actin and lamin A serve as cytoplasmic and nuclear protein loading controls. (D) Electrophoretic mobility shift assay of DNA binding to NF-κB in the nuclei extracted from BGC823 cells after DATS treatment for 12 h at different concentrations. Results in (A–D) are representative from at least two independent experiments. NF-κB, nuclear factor-kappaB; RT-PCR, reverse transcription–polymerase chain reaction.