FIG. 1.

Surgical model using bilayer cellularized PG-1. Schematic drawing (A) and representative image (B) of mouse model where two 6 mm diameter mouse skin punch biopsies were created on the back of immunosuppressed mice and skin substitutes were held in place on the wound using newly developed plastic domes. Animal wounds were randomly divided into three groups: control (no hydrogel), acellular hydrogel (hydrogel without cells), and cellularized hydrogel (bilayer hydrogel). The figure above shows the dome held in place using tissue glue (left) and nylon suture (right). In this experiment, domes were only secured with tissue glue. Color images available online at www.liebertpub.com/tea