FIG. 6.

Cellularized PG-1 increased skin cell proliferation, suppressed excessive immune response, and increased neovascularization. Representative images of immunohistochemistry stained images for (A–C) PCNA-positive cells, (E–G) F4/80-positive cells, and (I–K) ASM-positive cells. Arrows indicate cells positive for their respective stains. Scale bars represent 50 μm (40× magnification) for PCNA and F4/80 images and 100 μm (20× magnification) for ASM images. (D) Percentage of PCNA-positive cells were 9.93% ± 1.35%, 7.97% ± 2.54%, and 19.03% ± 2.40% (mean ± SEM) for control, acellular hydrogels, and cellular hydrogels respectively. (H) Percentage of F4/80-positive cells were 27.94% ± 4.19%, 1.22% ± 0.19%, and 2.84% ± 0.36% (mean ± SEM) for control, acellular hydrogels, and cellular hydrogels respectively. (L) Relative proportion of increased number of capillaries found in newly formed dermis was 5.81 ± 0.65 and 2.61 ± 0.34 for cellular hydrogels and acellular hydrogels respectively compared to control (1 ± 0.22) (mean ± SEM) (*p < 0.05, ***p < 0.001). ASM, alpha-smooth muscle antibody; PCNA, proliferating cell nuclear antigen. Color images available online at www.liebertpub.com/tea