Table 1.
Primers used for qPCR
| Gene | NCBI ID | EntrezGene ID | Primer | QuantiTect primer set | Amplified exons | Amplicon length, bp |
|---|---|---|---|---|---|---|
| YWHAZ | NM_001253805 (3,604 bp) NM_001253806 (3,329 bp) NM_001253807 (3,160 bp) NM_011740 (3,801 bp) | 22631 | Mm_Ywhaz_1_SG | QT00105350 | 3/4 | 99 |
| Caspases | ||||||
| Caspase-1 | NM_009807 (1,533 bp) | 12362 | Mm_Casp1_1_SG | QT00199458 | 6/7 | 101 |
| Caspase-2 | NM_007610 (3,463 bp) | 12366 | Mm_Casp2_1_SG | QT00108745 | 1/2 | 81 |
| Caspase-3 | NM_009810 (1,466 bp) | 12367 | Mm_Casp3_2_SG | QT01164779 | 3/4/5 | 150 |
| Caspase-4 | NM_007609 (1,433 bp) | 12363 | Mm_Casp4_1_SG | QT00096117 | 6/7 | 81 |
| Caspase-6 | NM_009811 (1,455 bp) | 12368 | Mm_Casp6_2_SG | QT00494921 | 6/7 | 125 |
| Caspase-7 | NM_007611 (2,350 bp) | 12369 | Mm_Casp7_1_SG | QT01058085 | 2/3 | 135 |
| Caspase-8 | NM_001080126 (2,412 bp) NM_009812 (2,585 bp) NM_001277926 (2,528 bp) | 12370 | Mm_Casp8_1_SG | QT00171437 | 7/8 | 74 |
| Caspase-9 | NM_015733 (3,896 bp) | 12371 | Mm_Casp9_1_SG | QT00133280 | 5/6/7 | 189 |
| Microglial phenotype | ||||||
| COX2 (Ptgs) | NM_011198 (4,150 bp) | 19225 | Mm_Ptgs2_1_SG | QT00165347 | 5/6 | 95 |
| iNOS (NOS2) | NM_010927 (3,990 bp) | 18126 | Mm_Nos2_va.1_SG | QT01535800 | 3/4 | 131 |
| IGF-1 | NM_001111274 (7,039 bp) NM_010512 (7,121 bp) | 16000 | Mm_Igf1_1_SG | QT00154469 | 3/4 | 114 |
| CD206 (MRC) | NM_008625 (5,322 bp) | 17533 | Mm_Mrc1_1_SG | QT00103012 | 28/29/30 | 132 |
| SOCS3 | NM_007707 (2,742 bp) | 12702 | Mm_Socs3_3_SG | QT02488990 | 90 | |
| IL-1ra | NM_001039701 (2,480 bp) NM_001159562 (2,409 bp) NM_031167 (2,464 bp) | 16181 | Mm_Il1rn_1_SG | QT00096054 | 2/3/4 | 116 |