Author response image 1. Caspase assay.
H1299 (A), SUM159 (B) and SAOS2 (C) cells were transfected with TAp63α mutants. Caspase-3 Antibody (Cell Signaling #9662) and PARP Antibody (Cell Signaling #9542) were used in A and B to detect full-length proteins and cleaved fragments (large fragment of caspase-3 (17 kDa) and large fragment of PARP (89 kDa)). Cleaved Caspase-3 Antibody (Cell Signaling #9662) was used in C to detect cleaved Caspase-3. No signal of any cleaved fragment was detected in dependency of TAp63α mutant transfection. Only inactive TAp63α (either wildtype or mutants bearing mutation F16A W20A L23A or R304Q (DNA-binding deficient)) reached high expression levels in the cells.