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. 2016 Apr 28;6(7):1012–1022. doi: 10.7150/thno.15095

Figure 2.

Figure 2

Nanoparticle diffusion in ECM-mimicking gels. (Scale bar: 200 μm). (A-C) The gels were prepared with 6.5 mg/mL of rat collagen I and 1 mg/mL of HA in capillary tubes. Ten μL of 1 mg/mL of nanoparticles (PLGA-green; RBC membrane-red) were added on the top of gels and incubated at 37ºC for 1 h before sample imaging. The separate labeling of membranes and nanoparticle cores, and the co-localization of two colors illustrated that the membranes did not separate from the particles. The activity of free or conjugated rHuPH20 was 50 U/mL. (D) Normalized NP fluorescence with diffusion distance in gels. Images were analyzed via ImageJ. Diffusion coefficients were obtained by fitting the data to the one-dimension diffusion model in MATLAB. Lines display theoretical intensity profiles (from top to bottom) for particles with diffusion coefficients of 4.11 × 10-8 cm2·s-1, 2.08 × 10-8 cm2·s-1 and 2.18 × 10-9 cm2·s-1.