Figure 3.
In vitro combination therapy.(a) Relative viabilities of 4T1 cells and HeLa cells after being incubated with various concentrations of PDA-ICG-PEG for 24 h. (b) Relative viabilities of 4T1 cells after being incubated with free DOX or PDA-ICG-PEG/DOX at various concentrations for 24 h. (c) Confocal fluorescence images of 4T1 cells incubated with PDA-ICG-PEG/DOX (or free DOX) with/without laser irradiation (808 nm, 0.8 W/cm2, 20 min). (d) Relative viabilities of 4T1 cells after being incubated with DOX, PDA-ICG-PEG, PDA-ICG-PEG/DOX with/without laser irradiation at different power density for 20 min. The cell viability test was conducted after further incubation for 24 h. The data are shown as mean ±standard deviation (SD) with at least triplicated measurements.