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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Int J Biol Macromol. 2015 Nov 19;83:433–441. doi: 10.1016/j.ijbiomac.2015.10.089

Figure 2. Analysis of purified α-syn, Hsc70 and its structural domains by size exclusion chromatography and SDS-PAGE (inset).

Figure 2

5 mM of these proteins were loaded on a superdex 75 column using high performance liquid chromatography (HPLC) and eluted as described under « Materials and methods ». Inset: 15 mg of each purified protein were analyzed by 12% SDS-PAGE as described under « Materials and methods ».