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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Int J Biol Macromol. 2015 Nov 19;83:433–441. doi: 10.1016/j.ijbiomac.2015.10.089

Figure 4. Aggregation of α-syn in presence of Hsc70.

Figure 4

A) Time course of α-syn aggregation monitored by ThT fluorescence in the absence (black) and presence of increasing amounts of Hsc70 (0.7 μM, bleu; 1.4 μM, brown; 3.5 μM, red and 7 μM, violet). The time-dependence of ThT fluorescence was fitted to a sigmoidal growth model as described under « Materials and methods ». Inset: percentage of Inhibition of α-syn aggregation at increasing ratios of [Hsc70]/[α-syn]. This % of inhibition was calculated at 52h taking the fluorescence of -syn in absence of Hsc70 as 0% of aggregation. ThT fluorescence results are the means of three independent experiments. B) AFM images of 70 μM α-syn at 0 h (sample 1), and 52h in presence of 7μM of Hsc70 (sample 2). C) DLS histograms of 70 μM α-syn at 0 h (sample 1) and 52h in presence of 7μM of Hsc70 (sample 2).