Table 1.
Kinetic parameters of α-syn aggregation in presence and absence of Hsc70 and its structural domains as determined from fluorescence assays. % of inhibition at 52 h is calculated from figure 5A and the dissociation constant Kd is obtained from the hyperbolic equation Y = Bmax*X/(Kd + X).
| X0 (t50) (h) | Tlag (h) | % of inhibition at 52h | Dissociation constant Kd (μM) | |
|---|---|---|---|---|
| α-syn | 20.95 | 11.75 | 0 | |
| Hsc70 (1-646) | 32.67 | 15.95 | 88 | 0.921 |
| Hsc70 (1-509) | 29.05 | 14.35 | 59 | 1.705 |
| Hsc70 (386-646) | 32.02 | 20.24 | 92 | 0.8217 |
| Hsc70 (386-509) | 28.64 | 13.91 | 56 | 1.467 |